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destination vectors pdest cmv c egfp  (Addgene inc)


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    Addgene inc destination vectors pdest cmv c egfp
    Figure 6. Assembly of endosomal-lysosomal organellar assembly (ELYSA) during migration and limited interaction with the acidification machinery. (A) Snapshots of a time-lapse observation for <t>EGFP</t> fluorescence of germinal vesicle (GV) oocytes injected with Lamp1-EGFP and V1A-mCherry mRNA are indicated. Maximum intensity projection of confocal images with a height of 40 μm (bottom half of the oocyte) are shown. Magnified perinuclear regions (right) are indicated by yellow boxes. Arrowheads indicate assemblies that adhere to each other in the subsequent frame. (B) Maximum intensity projection of deconvolved confocal images at an axial scan range of 80 µm is shown as Z-projection images. Magnified regions are indicated by yellow boxes (a, b) and the LAMP1-EGFP organelle regions are indicated by yellow dotted line.
    Destination Vectors Pdest Cmv C Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdest+cmv+c+egfp/pDEST-CMV-C-EGFP+(Plasmid+%23122844)/10__7554_slash_elife__99358-388-14-29
    Average 93 stars, based on 7 article reviews
    destination vectors pdest cmv c egfp - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Endosomal-lysosomal organellar assembly (ELYSA) structures coordinate lysosomal degradation systems through mammalian oocyte-to-embryo transition"

    Article Title: Endosomal-lysosomal organellar assembly (ELYSA) structures coordinate lysosomal degradation systems through mammalian oocyte-to-embryo transition

    Journal: eLife

    doi: 10.7554/elife.99358

    Figure 6. Assembly of endosomal-lysosomal organellar assembly (ELYSA) during migration and limited interaction with the acidification machinery. (A) Snapshots of a time-lapse observation for EGFP fluorescence of germinal vesicle (GV) oocytes injected with Lamp1-EGFP and V1A-mCherry mRNA are indicated. Maximum intensity projection of confocal images with a height of 40 μm (bottom half of the oocyte) are shown. Magnified perinuclear regions (right) are indicated by yellow boxes. Arrowheads indicate assemblies that adhere to each other in the subsequent frame. (B) Maximum intensity projection of deconvolved confocal images at an axial scan range of 80 µm is shown as Z-projection images. Magnified regions are indicated by yellow boxes (a, b) and the LAMP1-EGFP organelle regions are indicated by yellow dotted line.
    Figure Legend Snippet: Figure 6. Assembly of endosomal-lysosomal organellar assembly (ELYSA) during migration and limited interaction with the acidification machinery. (A) Snapshots of a time-lapse observation for EGFP fluorescence of germinal vesicle (GV) oocytes injected with Lamp1-EGFP and V1A-mCherry mRNA are indicated. Maximum intensity projection of confocal images with a height of 40 μm (bottom half of the oocyte) are shown. Magnified perinuclear regions (right) are indicated by yellow boxes. Arrowheads indicate assemblies that adhere to each other in the subsequent frame. (B) Maximum intensity projection of deconvolved confocal images at an axial scan range of 80 µm is shown as Z-projection images. Magnified regions are indicated by yellow boxes (a, b) and the LAMP1-EGFP organelle regions are indicated by yellow dotted line.

    Techniques Used: Migration, Fluorescence, Injection

    Related Articles

    Plasmid Preparation:

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling
    Article Snippet: .. EGFP-tagged RNF114 and SIRT6 were subcloned by Gateway Technology into pDEST-CMV-N-EGFP and pDEST-CMV-C-EGFP, respectively (this manuscript). pLNCX2 plasmid encodes EGFP (Empty Vector, Addgene). ..

    Article Title: Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
    Article Snippet: EGFP-tagged RNF114 and SIRT6 were subcloned by Gateway Technology into pDEST-CMV-N-EGFP and pDEST-CMV-C-EGFP, respectively (this manuscript). pLNCX2 plasmid encodes EGFP (Empty Vector, Addgene). .. EGFP-tagged RNF114 and SIRT6 were subcloned by Gateway Technology into pDEST-CMV-N-EGFP and pDEST-CMV-C-EGFP, respectively (this manuscript). pLNCX2 plasmid encodes EGFP (Empty Vector, Addgene). ..

    Article Title: Protein UFMylation regulates early events of ribosomal DNA double-stranded break response
    Article Snippet: DD-HA-ER-I-PpoI (a gift from Michael Kastan, Addgene: 49052) was subcloned into a pLX303 backbone vector (a gift from Mikko Taipale, Addgene: 154472) using In-Fusion cloning. .. The G418 (Geneticin) resistance gene was subcloned from pDEST-CMV-C-eGFP (a gift from Robin Ketteler, Addgene: 122844) and inserted into pLX303-DD-HA-ER-I-PpoI using In-Fusion cloning. eGFP vectors were cloned using Gateway cloning from pDONR221-eGFP (a gift from David Root, Addgene plasmid # 25899) and pFRT-TO-DEST (a gift from Dr. Thomas Tuschl, Addgene plasmid # 106348). ..

    Cloning:

    Article Title: Protein UFMylation regulates early events of ribosomal DNA double-stranded break response
    Article Snippet: DD-HA-ER-I-PpoI (a gift from Michael Kastan, Addgene: 49052) was subcloned into a pLX303 backbone vector (a gift from Mikko Taipale, Addgene: 154472) using In-Fusion cloning. .. The G418 (Geneticin) resistance gene was subcloned from pDEST-CMV-C-eGFP (a gift from Robin Ketteler, Addgene: 122844) and inserted into pLX303-DD-HA-ER-I-PpoI using In-Fusion cloning. eGFP vectors were cloned using Gateway cloning from pDONR221-eGFP (a gift from David Root, Addgene plasmid # 25899) and pFRT-TO-DEST (a gift from Dr. Thomas Tuschl, Addgene plasmid # 106348). ..

    Article Title: Calcyphosine is a microtubule-associated protein required for spindle formation and function
    Article Snippet: CAPS-FLAG was created by Gateway cloning the CAPS gene into pDEST-N-terminal FLAG (Addgene #18700), providing expression of N-terminally FLAG-tagged CAPS control of the cmv-promoter. .. CAPS-gfp was created by Gateway cloning the CAPS gene into pDEST-CMV-C-EGFP (Addgene #122844), to create an C-terminally-tagged gfp construct under control of the cmv promoter. .. For most experiments, 30pmol/ml CAPS siRNA hs.Ri.CAPS.13.3 (IDT) was transfected into cells using either RNAiMax (Invitrogen) or Lipofectamine 3000 (Invtrogen) with equivalent results.

    Clone Assay:

    Article Title: Protein UFMylation regulates early events of ribosomal DNA double-stranded break response
    Article Snippet: DD-HA-ER-I-PpoI (a gift from Michael Kastan, Addgene: 49052) was subcloned into a pLX303 backbone vector (a gift from Mikko Taipale, Addgene: 154472) using In-Fusion cloning. .. The G418 (Geneticin) resistance gene was subcloned from pDEST-CMV-C-eGFP (a gift from Robin Ketteler, Addgene: 122844) and inserted into pLX303-DD-HA-ER-I-PpoI using In-Fusion cloning. eGFP vectors were cloned using Gateway cloning from pDONR221-eGFP (a gift from David Root, Addgene plasmid # 25899) and pFRT-TO-DEST (a gift from Dr. Thomas Tuschl, Addgene plasmid # 106348). ..

    Construct:

    Article Title: Calcyphosine is a microtubule-associated protein required for spindle formation and function
    Article Snippet: CAPS-FLAG was created by Gateway cloning the CAPS gene into pDEST-N-terminal FLAG (Addgene #18700), providing expression of N-terminally FLAG-tagged CAPS control of the cmv-promoter. .. CAPS-gfp was created by Gateway cloning the CAPS gene into pDEST-CMV-C-EGFP (Addgene #122844), to create an C-terminally-tagged gfp construct under control of the cmv promoter. .. For most experiments, 30pmol/ml CAPS siRNA hs.Ri.CAPS.13.3 (IDT) was transfected into cells using either RNAiMax (Invitrogen) or Lipofectamine 3000 (Invtrogen) with equivalent results.

    Control:

    Article Title: Calcyphosine is a microtubule-associated protein required for spindle formation and function
    Article Snippet: CAPS-FLAG was created by Gateway cloning the CAPS gene into pDEST-N-terminal FLAG (Addgene #18700), providing expression of N-terminally FLAG-tagged CAPS control of the cmv-promoter. .. CAPS-gfp was created by Gateway cloning the CAPS gene into pDEST-CMV-C-EGFP (Addgene #122844), to create an C-terminally-tagged gfp construct under control of the cmv promoter. .. For most experiments, 30pmol/ml CAPS siRNA hs.Ri.CAPS.13.3 (IDT) was transfected into cells using either RNAiMax (Invitrogen) or Lipofectamine 3000 (Invtrogen) with equivalent results.



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    destination vectors pdest cmv c egfp  (Addgene inc)
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    Addgene inc destination vectors pdest cmv c egfp
    Figure 6. Assembly of endosomal-lysosomal organellar assembly (ELYSA) during migration and limited interaction with the acidification machinery. (A) Snapshots of a time-lapse observation for <t>EGFP</t> fluorescence of germinal vesicle (GV) oocytes injected with Lamp1-EGFP and V1A-mCherry mRNA are indicated. Maximum intensity projection of confocal images with a height of 40 μm (bottom half of the oocyte) are shown. Magnified perinuclear regions (right) are indicated by yellow boxes. Arrowheads indicate assemblies that adhere to each other in the subsequent frame. (B) Maximum intensity projection of deconvolved confocal images at an axial scan range of 80 µm is shown as Z-projection images. Magnified regions are indicated by yellow boxes (a, b) and the LAMP1-EGFP organelle regions are indicated by yellow dotted line.
    Destination Vectors Pdest Cmv C Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdest+cmv+c+egfp/pDEST-CMV-C-EGFP+(Plasmid+%23122844)/10__7554_slash_elife__99358-388-14-29
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    plasmid  (Addgene inc)
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    Figure 6. Assembly of endosomal-lysosomal organellar assembly (ELYSA) during migration and limited interaction with the acidification machinery. (A) Snapshots of a time-lapse observation for <t>EGFP</t> fluorescence of germinal vesicle (GV) oocytes injected with Lamp1-EGFP and V1A-mCherry mRNA are indicated. Maximum intensity projection of confocal images with a height of 40 μm (bottom half of the oocyte) are shown. Magnified perinuclear regions (right) are indicated by yellow boxes. Arrowheads indicate assemblies that adhere to each other in the subsequent frame. (B) Maximum intensity projection of deconvolved confocal images at an axial scan range of 80 µm is shown as Z-projection images. Magnified regions are indicated by yellow boxes (a, b) and the LAMP1-EGFP organelle regions are indicated by yellow dotted line.
    Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    destination vector pdest cmv egfp  (Addgene inc)
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    Addgene inc destination vector pdest cmv egfp
    Figure 6. Assembly of endosomal-lysosomal organellar assembly (ELYSA) during migration and limited interaction with the acidification machinery. (A) Snapshots of a time-lapse observation for <t>EGFP</t> fluorescence of germinal vesicle (GV) oocytes injected with Lamp1-EGFP and V1A-mCherry mRNA are indicated. Maximum intensity projection of confocal images with a height of 40 μm (bottom half of the oocyte) are shown. Magnified perinuclear regions (right) are indicated by yellow boxes. Arrowheads indicate assemblies that adhere to each other in the subsequent frame. (B) Maximum intensity projection of deconvolved confocal images at an axial scan range of 80 µm is shown as Z-projection images. Magnified regions are indicated by yellow boxes (a, b) and the LAMP1-EGFP organelle regions are indicated by yellow dotted line.
    Destination Vector Pdest Cmv Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    pdest cmv cegfp  (Addgene inc)
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    Figure 6. Assembly of endosomal-lysosomal organellar assembly (ELYSA) during migration and limited interaction with the acidification machinery. (A) Snapshots of a time-lapse observation for <t>EGFP</t> fluorescence of germinal vesicle (GV) oocytes injected with Lamp1-EGFP and V1A-mCherry mRNA are indicated. Maximum intensity projection of confocal images with a height of 40 μm (bottom half of the oocyte) are shown. Magnified perinuclear regions (right) are indicated by yellow boxes. Arrowheads indicate assemblies that adhere to each other in the subsequent frame. (B) Maximum intensity projection of deconvolved confocal images at an axial scan range of 80 µm is shown as Z-projection images. Magnified regions are indicated by yellow boxes (a, b) and the LAMP1-EGFP organelle regions are indicated by yellow dotted line.
    Pdest Cmv Cegfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pdest cmv c egfp  (Addgene inc)
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    Figure 6. Assembly of endosomal-lysosomal organellar assembly (ELYSA) during migration and limited interaction with the acidification machinery. (A) Snapshots of a time-lapse observation for <t>EGFP</t> fluorescence of germinal vesicle (GV) oocytes injected with Lamp1-EGFP and V1A-mCherry mRNA are indicated. Maximum intensity projection of confocal images with a height of 40 μm (bottom half of the oocyte) are shown. Magnified perinuclear regions (right) are indicated by yellow boxes. Arrowheads indicate assemblies that adhere to each other in the subsequent frame. (B) Maximum intensity projection of deconvolved confocal images at an axial scan range of 80 µm is shown as Z-projection images. Magnified regions are indicated by yellow boxes (a, b) and the LAMP1-EGFP organelle regions are indicated by yellow dotted line.
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    caps gene  (Addgene inc)
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    <t>CAPS</t> is associated with the mitotic spindle A. Cultured human SKBR3 cells were fixed and stained with antibodies against CAPS (red) and alpha-tubulin (green); DAPI is shown in blue. Fluorescent immunostaining shows CAPS along microtubules between the spindle poles at all stages of mitosis. B) U251-MG cells transfected <t>with</t> <t>CAPS-gfp</t> show a pattern of CAPS localization similar to that seen with CAPS antibody. C) Metaphase cell from (A) showing blow up of area in square (C’). The localization of CAPS to the spindle is limited to central microtubules; CAPS is not found along astral microtubules (arrow). D. In interphase cells, CAPS does not appear to colocalize with cytoplasmic microtubules; D’ blow up of square in D. Staining as in (A). Images are max Z-projections of confocal stacks. Scale bars are 5um in all pictures.
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    Image Search Results


    Figure 6. Assembly of endosomal-lysosomal organellar assembly (ELYSA) during migration and limited interaction with the acidification machinery. (A) Snapshots of a time-lapse observation for EGFP fluorescence of germinal vesicle (GV) oocytes injected with Lamp1-EGFP and V1A-mCherry mRNA are indicated. Maximum intensity projection of confocal images with a height of 40 μm (bottom half of the oocyte) are shown. Magnified perinuclear regions (right) are indicated by yellow boxes. Arrowheads indicate assemblies that adhere to each other in the subsequent frame. (B) Maximum intensity projection of deconvolved confocal images at an axial scan range of 80 µm is shown as Z-projection images. Magnified regions are indicated by yellow boxes (a, b) and the LAMP1-EGFP organelle regions are indicated by yellow dotted line.

    Journal: eLife

    Article Title: Endosomal-lysosomal organellar assembly (ELYSA) structures coordinate lysosomal degradation systems through mammalian oocyte-to-embryo transition

    doi: 10.7554/elife.99358

    Figure Lengend Snippet: Figure 6. Assembly of endosomal-lysosomal organellar assembly (ELYSA) during migration and limited interaction with the acidification machinery. (A) Snapshots of a time-lapse observation for EGFP fluorescence of germinal vesicle (GV) oocytes injected with Lamp1-EGFP and V1A-mCherry mRNA are indicated. Maximum intensity projection of confocal images with a height of 40 μm (bottom half of the oocyte) are shown. Magnified perinuclear regions (right) are indicated by yellow boxes. Arrowheads indicate assemblies that adhere to each other in the subsequent frame. (B) Maximum intensity projection of deconvolved confocal images at an axial scan range of 80 µm is shown as Z-projection images. Magnified regions are indicated by yellow boxes (a, b) and the LAMP1-EGFP organelle regions are indicated by yellow dotted line.

    Article Snippet: The amplicon was then subcloned into the entry vector pDONR221 and transferred into the destination vectors pDEST- CMV- C- EGFP (Agrotis et al., 2019) (a gift from Robin Ketteler [Addgene 0 200 400 600 800 1000 1200 0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000 11000 0 3 5 8 10 13 15 18 20 23 25 28 30 33 35 38 40 43 45 48 LysoSensor Magic Red 0 200 400 600 800 1000 1200 0 1000 2000 3000 4000 5000 6000 7000 8000 9000 0 2 4 6 9 11131517192224262830323537394143 LysoSensor Magic Red A GV (nuclear) media Isolated punctaAssembly(perinuclear) Assembly In te ns i es Distance from origin (μm) GV MII Isolated puncta media media Assembly (cor cal) Assembly (cor cal) In te ns i es MIIGV BF Ly so Se ns or M ag ic Re d B M er ge BF 20 μm B Satouh et al. eLife 2024;13:RP99358.

    Techniques: Migration, Fluorescence, Injection

    CAPS is associated with the mitotic spindle A. Cultured human SKBR3 cells were fixed and stained with antibodies against CAPS (red) and alpha-tubulin (green); DAPI is shown in blue. Fluorescent immunostaining shows CAPS along microtubules between the spindle poles at all stages of mitosis. B) U251-MG cells transfected with CAPS-gfp show a pattern of CAPS localization similar to that seen with CAPS antibody. C) Metaphase cell from (A) showing blow up of area in square (C’). The localization of CAPS to the spindle is limited to central microtubules; CAPS is not found along astral microtubules (arrow). D. In interphase cells, CAPS does not appear to colocalize with cytoplasmic microtubules; D’ blow up of square in D. Staining as in (A). Images are max Z-projections of confocal stacks. Scale bars are 5um in all pictures.

    Journal: bioRxiv

    Article Title: Calcyphosine is a microtubule-associated protein required for spindle formation and function

    doi: 10.1101/2023.12.29.573632

    Figure Lengend Snippet: CAPS is associated with the mitotic spindle A. Cultured human SKBR3 cells were fixed and stained with antibodies against CAPS (red) and alpha-tubulin (green); DAPI is shown in blue. Fluorescent immunostaining shows CAPS along microtubules between the spindle poles at all stages of mitosis. B) U251-MG cells transfected with CAPS-gfp show a pattern of CAPS localization similar to that seen with CAPS antibody. C) Metaphase cell from (A) showing blow up of area in square (C’). The localization of CAPS to the spindle is limited to central microtubules; CAPS is not found along astral microtubules (arrow). D. In interphase cells, CAPS does not appear to colocalize with cytoplasmic microtubules; D’ blow up of square in D. Staining as in (A). Images are max Z-projections of confocal stacks. Scale bars are 5um in all pictures.

    Article Snippet: CAPS-gfp was created by Gateway cloning the CAPS gene into pDEST-CMV-C-EGFP (Addgene #122844), to create an C-terminally-tagged gfp construct under control of the cmv promoter.

    Techniques: Cell Culture, Staining, Immunostaining, Transfection

    Overexpression of CAPS does not alter cell proliferation or apoptosis. U251MG cells transfected with CAPS-FLAG were evaluated for changes in mitotic index or apoptosis. A) No differences in mitotic index were observed between mock transfected cells and those transfected with CAPS-FLAG B) CAPS-FLAG did not alter the percentage of apoptotic cells as measured by flow cytometry (as in ). C) MTT assay shows no significant difference in proliferation between cells transfected with CAPS-FLAG and control (cmv-gfp) at any serum concentration (0%, 0.5%, 1%).

    Journal: bioRxiv

    Article Title: Calcyphosine is a microtubule-associated protein required for spindle formation and function

    doi: 10.1101/2023.12.29.573632

    Figure Lengend Snippet: Overexpression of CAPS does not alter cell proliferation or apoptosis. U251MG cells transfected with CAPS-FLAG were evaluated for changes in mitotic index or apoptosis. A) No differences in mitotic index were observed between mock transfected cells and those transfected with CAPS-FLAG B) CAPS-FLAG did not alter the percentage of apoptotic cells as measured by flow cytometry (as in ). C) MTT assay shows no significant difference in proliferation between cells transfected with CAPS-FLAG and control (cmv-gfp) at any serum concentration (0%, 0.5%, 1%).

    Article Snippet: CAPS-gfp was created by Gateway cloning the CAPS gene into pDEST-CMV-C-EGFP (Addgene #122844), to create an C-terminally-tagged gfp construct under control of the cmv promoter.

    Techniques: Over Expression, Transfection, Flow Cytometry, MTT Assay, Control, Concentration Assay